News

Osteoarthritis is a common joint disease. The joint pain it causes seriously affects the daily life of patients and has always been a key topic in medical research. In order to further explore its pathogenesis, professional researchers used a fully automatic sample grinder to efficiently grind cartilage tissue, extract key proteins, and compare the gene expression differences between diseased mice and normal mice at the genetic level. The researchers successfully screened out biomarkers related to osteoarthritis, providing an important scientific basis for the early diagnosis and precise treatment of the disease.

What are the advantages of grinding cartilage with a fully automatic grinder?

We know that cartilage is a tough tissue rich in matrix components, mainly composed of collagen and proteoglycans, with extremely low cell density and lack of blood vessels and nerve supply. This structural feature makes it difficult to fully homogenize cartilage samples during processing, and insufficient sample processing may lead to reduced data reliability and reproducibility in subsequent experiments.

Extracting high-quality proteins and nucleic acids from cartilage tissue is crucial for subsequent analysis. Efficient pretreatment can protect the integrity of biomolecules and reduce degradation and loss caused by excessive grinding or uneven treatment, thereby ensuring the accuracy of analytical results.

High efficiency

Traditional manual grinding is not only time-consuming and labor-intensive, but also difficult to fully homogenize the sample, so it is prone to human error. With the help of a fully automatic grinder, cartilage can be quickly and evenly ground into powder in a short time, significantly improving sample processing efficiency while ensuring consistency between samples, saving time for subsequent experiments.

High Fineness

The cartilage ground by this grinder can be fine and uniform, providing high-quality samples for subsequent experimental analysis. Uniform sample processing can significantly improve the accuracy and reliability of experimental results.

Zero Contamination

When using a grinder for sample pretreatment, using a disposable grinding tube can effectively avoid direct contact between the sample and the instrument, thereby preventing external contamination to the greatest extent. In addition, the disposable grinding tube also reduces the risk of sample cross-contamination, ensures the purity of the sample and the reliability of the experimental results, and simplifies the cleaning process to improve experimental efficiency.

Repeatability

The grinder supports the function of saving grinding parameters, which can accurately record key parameters such as speed, time, frequency, etc. By reusing the same parameter settings, it can ensure the consistency of sample processing in different experimental batches and obtain highly similar experimental results. This repeatability not only helps to verify scientific research results, but also provides a reliable technical guarantee for the promotion and application of experimental data.

Below we will share in detail the experimental process and key steps of grinding cartilage samples using a fully automatic grinder. Through this experiment, we demonstrated how to use advanced equipment to achieve efficient and uniform sample processing and lay a solid foundation for subsequent experimental analysis. I hope these experiences can provide reference and inspiration for your related research.

Experimental equipment: Welso fully automatic sample grinder

Purpose of the experiment: Grind the cartilage tissue of diseased mice, extract high-quality proteins, and analyze the expression level of inflammatory factors. By comparing with normal mice, we can deeply explore the pathogenesis of osteoarthritis and provide a scientific basis for disease diagnosis and treatment.

Experimental materials: diseased mice, protein extraction kit, grinding tubes, adapters, grinding beads, etc.

Experimental steps:

Sample collection: dissect mice and accurately take out cartilage tissue, minimize damage to tissues and ensure sample integrity.

Sample preparation: place cartilage tissue in a disposable grinding tube, add appropriate amount of protein extraction reagent and grinding beads to ensure that the sample is fully homogenized during the grinding process.

Precooling: put the grinding tube containing the sample and the adapter into a -80℃ refrigerator for precooling, usually for 30 minutes to 1 hour, to avoid the heat generated by friction during the grinding process which affects protein activity.

Grinding: put the precooled grinding tube into the fully automatic grinder, set the grinding parameters (such as grinding speed, time, frequency, etc.) according to the experimental requirements, and ensure the consistency and fineness of sample processing.

Grinding completion: after grinding, carefully take out the grinding tube, check the sample status, and confirm that the cartilage tissue has been fully ground.

Subsequent processing: the ground sample is used for protein extraction and subsequent experimental analysis, such as the expression detection of inflammatory factors and comparative analysis of gene expression levels.

Experimental results:

Left is before grinding (extraction reagent added), right is after grinding (reagent has been sucked out)

As can be seen from the figure, the cartilage sample was fully ground and the protein was successfully extracted. Next, the extracted protein was analyzed in depth using proteomics technology to compare the differential expression of proteins in the cartilage of diseased and normal mice, revealing possible biomarkers and potential therapeutic targets. This process provides important clues for the study of the mechanism of osteoarthritis and the development of targeted treatment strategies.

In summary, the fully automatic sample rapid grinder can efficiently process various types of samples in a short time with its advanced design and powerful performance to meet different scientific research needs. Whether it is soil, plant, animal tissue, or microbial and paleontological specimens, the device can provide consistent and high-quality grinding results.