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One of the most important tools in cell biology research is the cell stainer, which is suitable for the filtration of stem cells and primary cells, and is also necessary for flow cytometry sorting experiments. Filtration of cell samples is key to the accuracy of the results, and the use of cell strainer also allows for the removal of possible cell clumps or debris, which is also more convenient and faster than traditional gauze filtration.

This article introduces the cell sieve in an all-round way, hoping to help laboratory researchers better use and choose it.

Size and Application

There are three main sizes of cell strainers: 40, 70, and 100μm, where the size refers to the membrane pore size.

40 μm is suitable for filtration of mouse B cells, intestinal epithelial stem cells, MSCs and other cells.

70 μm is suitable for lymph node cells of mice, bone marrow cells of wound tissue, etc.

100 μm is suitable for filtering larger cell sizes, such as rat glomerular cells, heart cells, etc.

Cell strainers with different pore sizes are identified with different colors, which is convenient for users to distinguish. 40 μm is blue, 70 μm is white, 100 μm is yellow, of which 70 μm is the most commonly used.

It is also stackable with different pore sizes, allowing continuous filtration from large to small pore sizes. The handle design is convenient for the operator to operate aseptically.

Material

Welso's cell sieve is mainly composed of polypropylene PP material and medical nylon mesh, which has low resistance, can be cleaned repeatedly, has good corrosion resistance, and is a kind of cost-effective material.

Packaging

Each one is individually packaged and electron beam sterilized to ensure DNase, RNase, and pyrogen-free.

Experiment Examples:

In order to better understand the use of cell strainers, we have compiled experimental cases of single-cell suspension preparation in mouse spleen for reference only.

The spleen is an immune organ, rich in a large number of lymphocytes and macrophages, in immunology, such as regulatory T cells, helper T cells, macrophages and other popular research, are related to the spleen, so how to use the cell strainer to obtain better activity of spleen single cells?

Experiment Procedure

●Obtain fresh mouse spleens, which are oval in shape and can be pulled directly with tweezers. Place the mouse spleen in a pre-chilled petri dish or a moderately sized centrifuge tube with an appropriate amount of HBSS (Hanks' Balanced Salt Solution) buffer and carefully chop the spleen using surgical or ophthalmic scissors.

●Place a 70 μm cell strainer on top of a 50 mL centrifuge tube, and using a disposable pipette, transfer the shredded spleen to the cell strainer and gently mash or crush it to pass through the sieve. Rinse with 5–10 mL of PBS if necessary. Repeat the above steps for verification with a 1 mL syringe, and if the cell suspension through the sieve can be pipetted repeatedly with a syringe (with needle) without hindrance, the single-cell suspension is considered complete.

Note

The disposable cell strainer has been sterilized and it is not recommended to wash and disinfect it, as it may cause uneven strainers and affect the results of the next experiment.

This gadget is used in organ culture, tissue transport or transplantation, and filtration of stem cells and primary cells. It is indispensable in the laboratory. If you need it, please contact Welso and we will provide you with the best price plan.